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包被用人內(nèi)肽-2(EM-2)ELISA試劑盒抗原:用于包被固相載體的抗原按其來源不同可分為天然抗原、重組抗原和合成多肽抗原三大類。天然抗原可取自動物組織、微生物培養(yǎng)物等,須經(jīng)提取純化才能作包被用。
包被用抗體:包被固相載體的抗體應具有高親和力和高特異性,可取材于抗血清或含單克隆抗體的腹水或培養(yǎng)液。如免疫用抗原中含有雜質(zhì)(即便是極微量的),在抗血清中將出現(xiàn)雜抗體,必須除去(可用吸收法)后才能用于ELISA,以保證試驗的特異性。抗血清不能直接用于包被,應先提取IgG,通常采用硫酸銨鹽析和Sephadex凝膠過濾法。一般經(jīng)硫酸銨鹽析粗提的IgG已可用于包被,高度純化的IgG性質(zhì)不穩(wěn)定。如需用高親和力的抗體包被以提高試驗的敏感性,則可采用親和層析法以除去抗血清中含量較多的非特異性IgG。腹水中單抗的濃度較高,特異性亦較強,因此不需要作吸收和親和層析處理,一般可將腹水作適當稀釋后直接包被,必要時也可用純化的IgG。應用單抗包被時應注意,一種單抗僅針對一種抗原決定簇,在某些情況下,用多種單抗混合包被,可取得更好的效果。
109371 再生胰島衍生蛋白1β(REG1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Regenerating Islet Derived Protein 1 Beta (REG1b) 48T/96T Mus musculus (Mouse)
109372 睪丸陰離子轉(zhuǎn)運蛋白1(TAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Testis Anion Transporter 1 (TAT1) 48T/96T Homo sapiens (Human)
109373 鈉/葡萄糖協(xié)同轉(zhuǎn)運蛋白2(SGLT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Sodium/Glucose Cotransporter 2 (SGLT2) 48T/96T Rattus norvegicus (Rat)
109374 鈉/葡萄糖協(xié)同轉(zhuǎn)運蛋白1(SGLT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Sodium/Glucose Cotransporter 1 (SGLT1) 48T/96T Mus musculus (Mouse)
109375 重肽神經(jīng)絲蛋白(NEFH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neurofilament, Heavy Polypeptide (NEFH) 48T/96T Rattus norvegicus (Rat)
109376 腎上腺素能受體β3(ADRβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Adrenergic Receptor Beta 3 (ADRb3) 48T/96T Rattus norvegicus (Rat)
109377 腎上腺素能受體β2(ADRβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Adrenergic Receptor Beta 2 (ADRb2) 48T/96T Rattus norvegicus (Rat)
109378 核受體相關蛋白1(NURR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nuclear Receptor Related Protein 1 (NURR1) 48T/96T Homo sapiens (Human)
109379 腫瘤壞死因子配體超家族成員9(TNFSF9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) 48T/96T Homo sapiens (Human)
109380 90kDa熱休克蛋白αB1(HSP90αB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) 48T/96T Bos taurus; Bovine (Cattle)
109381 5-羥色胺受體2A(HTR2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for 5-Hydroxytryptamine Receptor 2A (HTR2A) 48T/96T Homo sapiens (Human)
109382 肌球蛋白重鏈6(MYH6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) 48T/96T Homo sapiens (Human)
109383 肌球蛋白重鏈7(MYH7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) 48T/96T Homo sapiens (Human)
109384 肌球蛋白重鏈4(MYH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myosin Heavy Chain 4, Skeletal Muscle (MYH4) 48T/96T Homo sapiens (Human)
109385 蛋白酶體亞基α1(PSMα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Proteasome Subunit Alpha Type 1 (PSMa1) 48T/96T Homo sapiens (Human)
109386 20S-蛋白酶體(20S-PSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for 20S-Proteasome (20S-PSM) 48T/96T Mus musculus (Mouse)
109387 20S-蛋白酶體(20S-PSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for 20S-Proteasome (20S-PSM) 48T/96T Homo sapiens (Human)
109388 饑餓素/肥胖抑制素前激素原(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ghrelin/Obestatin Preprohormone (GHRL) 48T/96T Rattus norvegicus (Rat)
109389 骨骼肌慢肌肌鈣蛋白T(TNNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1) 48T/96T Rattus norvegicus (Rat)
109390 骨骼肌慢肌肌鈣蛋白T(TNNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1) 48T/96T Mus musculus (Mouse)
109391 輕肽鐵蛋白(FTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ferritin, Light Polypeptide (FTL) 48T/96T Mus musculus (Mouse)
109392 甲狀旁腺素受體1(PTHR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Parathyroid Hormone Receptor 1 (PTHR1) 48T/96T Homo sapiens (Human)
109393 組蛋白脫乙?;?(HDAC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Histone Deacetylase 1 (HDAC1) 48T/96T Mus musculus (Mouse)
109394 過氧化物酶體增殖物激活受體δ(PPARδ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Peroxisome Proliferator Activated Receptor Delta (PPARd) 48T/96T Mus musculus (Mouse)
109395 白介素22(IL22)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interleukin 22 (IL22) 48T/96T Bos taurus; Bovine (Cattle)
109396 殺傷細胞免疫球蛋白樣受體2DS4(KIR2DS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) 48T/96T Homo sapiens (Human)
109397 腺苷酸環(huán)化酶10(ADCY10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Adenylate Cyclase 10, Soluble (ADCY10) 48T/96T Mus musculus (Mouse)
109398 Bcl2關聯(lián)X蛋白(Bax)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Bcl2 Associated X Protein (Bax) 48T/96T Bos taurus; Bovine (Cattle)
109399 早老素2(PS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Presenilin 2 (PS2) 48T/96T Rattus norvegicus (Rat)
109400 胞外5'-核苷酸酶(NT5E)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for 5'-Nucleotidase, Ecto (NT5E) 48T/96T Rattus norvegicus (Rat)
109401 心型脂肪酸結(jié)合蛋白(FABP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) 48T/96T Mus musculus (Mouse)
109402 整合素α2(ITGα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Integrin Alpha 2 (ITGa2) 48T/96T Rattus norvegicus (Rat)
109403 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Parathyroid Hormone Related Protein (PTHrP) 48T/96T Bos taurus; Bovine (Cattle)
109404 B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) 48T/96T Bos taurus; Bovine (Cattle)
109405 肌球蛋白重鏈2(MYH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) 48T/96T Homo sapiens (Human)
109406 細胞程序性死亡蛋白1(PDCD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Programmed Cell Death Protein 1 (PDCD1) 48T/96T Homo sapiens (Human)
109407 補體受體2(CR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Complement Receptor 2 (CR2) 48T/96T Rattus norvegicus (Rat)
109408 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Caspase 3 (CASP3) 48T/96T Bos taurus; Bovine (Cattle)
109409 銅伴侶超氧化物歧化酶(SOD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4) 48T/96T Homo sapiens (Human)
109410 抑制素βC(INHβC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Inhibin Beta C (INHbC) 48T/96T Mus musculus (Mouse)
109411 短腭肺鼻腔上皮癌關聯(lián)蛋白3(SPLUNC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3) 48T/96T Homo sapiens (Human)
109412 短腭肺鼻腔上皮癌關聯(lián)蛋白2(SPLUNC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) 48T/96T Homo sapiens (Human)
109413 胎盤特異性蛋白9(PLAC9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Placenta Specific Protein 9 (PLAC9) 48T/96T Homo sapiens (Human)
109414 棘皮動物微管關聯(lián)蛋白樣蛋白2(EML2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) 48T/96T Homo sapiens (Human)
109415 鈉/鉀離子轉(zhuǎn)運ATP酶β4肽(ATP1β4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) 48T/96T Rattus norvegicus (Rat)
109416 粘蛋白12(MUC12)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Mucin 12, Cell Surface Associated (MUC12) 48T/96T Homo sapiens (Human)
109417 T-框蛋白21(TBX21)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for T-Box Protein 21 (TBX21) 48T/96T Homo sapiens (Human)
109418 無翅型MMTV整合位點家族成員9B(WNT9B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) 48T/96T Homo sapiens (Human)
109419 肌球蛋白重鏈7B(MYH7B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) 48T/96T Homo sapiens (Human)
109420 含Toll白細胞介素1受體域銜接蛋白(TIRAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) 48T/96T Homo sapiens (Human)
109421 絲氨酸蛋白酶50(PRSS50)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protease, Serine 50 (PRSS50) 48T/96T Homo sapiens (Human)
109422 赫曼斯基普德拉克綜合征蛋白6(HPS6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Hermansky Pudlak Syndrome Protein 6 (HPS6) 48T/96T Homo sapiens (Human)
109423 含SET域蛋白7(SETD7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for SET Domain Containing Protein 7 (SETD7) 48T/96T Homo sapiens (Human)
109424 類固醇5α還原酶2(SRD5α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) 48T/96T Homo sapiens (Human)
109425 含RCC1和BTB域蛋白2(RCBTB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for RCC1 And BTB Domain Containing Protein 2 (RCBTB2) 48T/96T Homo sapiens (Human)
109426 無翅型MMTV整合位點家族成員4(WNT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4) 48T/96T Homo sapiens (Human)
109427 FK506結(jié)合蛋白樣蛋白(FKBPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for FK506 Binding Protein Like Protein (FKBPL) 48T/96T Mus musculus (Mouse)
109428 含亮氨酸豐富重復蛋白3C(LRRC3C)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) 48T/96T Homo sapiens (Human)
109429 端粒保護1同源物(POT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protection Of omeres 1 Homolog (POT1) 48T/96T Homo sapiens (Human)
109430 亮氨酸豐富重復FLII相互作用蛋白1(LRRFIP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) 48T/96T Homo sapiens (Human)
將抗原或抗體固定在過程稱為包被(coating)。換言之,包被即是抗原或抗體結(jié)合到固相載體表面的過程,脂類物質(zhì)無法與固相載體結(jié)合,可將其在有機溶劑(例如乙醇)中溶解后加入板孔中,開蓋置冰箱過夜或冷風吹干,待酒精揮發(fā)后,讓脂質(zhì)自然干固在固相表面??剐牧字贵w的ELISA試劑一般采用這種包被方式。
人內(nèi)肽-2(EM-2)ELISA試劑盒
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