更多人ELISA試劑盒
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包被用人趨化因子(fractalkine/CX3CL1)ELISA試劑盒抗原:用于包被固相載體的抗原按其來源不同可分為天然抗原、重組抗原和合成多肽抗原三大類。天然抗原可取自動(dòng)物組織、微生物培養(yǎng)物等,須經(jīng)提取純化才能作包被用。
包被用抗體:包被固相載體的抗體應(yīng)具有高親和力和高特異性,可取材于抗血清或含單克隆抗體的腹水或培養(yǎng)液。如免疫用抗原中含有雜質(zhì)(即便是極微量的),在抗血清中將出現(xiàn)雜抗體,必須除去(可用吸收法)后才能用于ELISA,以保證試驗(yàn)的特異性??寡宀荒苤苯佑糜诎?,應(yīng)先提取IgG,通常采用硫酸銨鹽析和Sephadex凝膠過濾法。一般經(jīng)硫酸銨鹽析粗提的IgG已可用于包被,高度純化的IgG性質(zhì)不穩(wěn)定。如需用高親和力的抗體包被以提高試驗(yàn)的敏感性,則可采用親和層析法以除去抗血清中含量較多的非特異性IgG。腹水中單抗的濃度較高,特異性亦較強(qiáng),因此不需要作吸收和親和層析處理,一般可將腹水作適當(dāng)稀釋后直接包被,必要時(shí)也可用純化的IgG。應(yīng)用單抗包被時(shí)應(yīng)注意,一種單抗僅針對(duì)一種抗原決定簇,在某些情況下,用多種單抗混合包被,可取得更好的效果。
108891 人FASL Human FASL ELISA Kit 96T/48T
108892 人酸性成纖維細(xì)胞生長(zhǎng)因子(FGF1) Human Fibroblast growth factor acidic ELISA Kit 96T/48T
108893 人堿性成纖維細(xì)胞生長(zhǎng)因子-4(FGF4) Human Fibroblast growth factor 4 ELISA Kit 96T/48T
108894 人堿性成纖維細(xì)胞生長(zhǎng)因子-6(FGF6) Human Fibroblast growth factor 6 ELISA Kit 96T/48T
108895 人堿性成纖維細(xì)胞生長(zhǎng)因子9(FGF9) Human FGF9 ELISA Kit 96T/48T
108896 人纖維連接蛋白(FN) Human Fibronectin ELISA Kit 96T/48T
108897 人Flt-3配體(Flt-3 ligand) Human Flt-3 ligand ELISA Kit 96T/48T
108898 人Flt-3 Human Flt-3 ELISA Kit 96T/48T
108899 人Fractalkine Human Fractalkine ELISA Kit 96T/48T
108900 人中性粒細(xì)胞趨化蛋白-2(GCP-2) Human Granulocyte Chemotactic Protein-2 ELISA Kit 96T/48T
108901 人粒細(xì)胞克隆刺激因子(G-CSF) Human G-CSF ELISA Kit 96T/48T
108902 人膠質(zhì)細(xì)胞系來源的神經(jīng)營(yíng)養(yǎng)因子(GDNF) Human Glial Cell-line derived Neurotrophic Factor ELISA Kit 96T/48T
108903 人生長(zhǎng)因子(GH) Human Growth hormone ELISA Kit 96T/48T
108904 人粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF) Human Granulocyte-Macrophage Colony Stimulating Factor ELISA Kit 96T/48T
108905 人gp130 Human gp130 soluble fragment ELISA KIT 96T/48T
108906 人肝細(xì)胞生長(zhǎng)因子(HGF)ELISA kit Human Hepatocyte growth factor ELISA kit 96T/48T
108907 人細(xì)胞間粘附分子1(ICAM-1)(CD54)ELISA KIT Human Intercellular adhesion molecular 1 ELISA KIT 96T/48T
108908 人干擾素γ (IFNγ)ELISA Kit Human Interferon gamma ELISA Kit 96T/48T
108909 人胰島素樣生長(zhǎng)因子1(IGF-1)ELISA Kit Human Insulin-like growth factor 1, IGF-1 ELISA Kit 96T/48T
108910 人胰島素樣生長(zhǎng)因子2(IGF-2) Human Insulin-like growth factor 2, IGF-II ELISA Kit 96T/48T
108911 人胰島素樣生長(zhǎng)因子結(jié)合蛋白1(IGFBP1) Human Insulin-like growth factor binding protein 1 ELISA Kit 96T/48T
108912 人胰島素樣生長(zhǎng)因子結(jié)合蛋白2(IGFBP2) Human Insulin-like growth factor binding protein 2, IGFBP2 ELISA KIT 96T/48T
108913 人胰島素樣生長(zhǎng)因子結(jié)合蛋白3(IGFBP3) Human Insulin-like growth factor binding protein 3, IGFBP3 ELISA KIT 96T/48T
108914 人胰島素樣生長(zhǎng)因子結(jié)合蛋白4(IGFBP4) Human Insulin-like growth factor binding protein 4, IGFBP4 ELISA kit 96T/48T
108915 人白介素10(IL-10)ELISA KIT Human IL-10 ELISA KIT 96T/48T
108916 人白介素-11(IL-11) Human Interleukin-11(IL-11)ELISA KIT 96T/48T
108917 人白介素-12(IL-12)(P40) Human Interleukin-12(IL-12)ELISA KIT 96T/48T
108918 人白介素-12(IL-12)(P70) Human Interleukin-12,IL-12(P70)ELISA KIT 96T/48T
108919 人白介素-13(IL-13) Human Interleukin-13 ,IL-13 ELISA KIT 96T/48T
108920 人白介素-15(IL-15) Human Interleukin-15 ,IL-15 ELISA KIT 96T/48T
108921 人白介素-16(IL-16) Human Interleukin-16 ELISA KIT 96T/48T
108922 人白介素1可溶性受體Ⅰ(IL-1sRⅠ) Human Interleukin-1 receptorⅠ,IL-1sRⅠ ELISA kit 96T/48T
108923 人白介素1可溶性受體Ⅱ(IL-1sRⅡ) Human Interleukin-1 receptorⅡ,IL-1sRⅡ ELISA kit 96T/48T
108924 人白介素1α (IL-1α ) Human Interleukin-1α, IL-1α ELISA Kit 96T/48T
108925 人白介素1可溶性受體I(IL-1sRI) Human Interleukin-1 receptorI(IL-1sRI) ELISA kit 96T/48T
108926 人白介素1可溶性受體Ⅱ(IL-1sRⅡ) Human Interleukin-1 receptorⅡ,IL-1sRⅡ ELISA kit 96T/48T
108927 人白介素1α (IL-1α ) Human Interleukin-1α, IL-1α ELISA Kit 96T/48T
108928 人白介素2(IL-2) Human Interleukin-2 ELISA kit 96T/48T
108929 人白介素2可溶性受體α鏈(CD25) (IL-2sRα ) Human Interleukin-2 soluble receptor alpha chain,IL-2sRα ELISA kit 96T/48T
108930 人白介素2可溶性受體β鏈(IL-2sRβ ) Human Interleukin-2 soluble receptor alpha chain,IL-2sRβ ELISA kit 96T/48T
108931 人白介素3(IL-3) Human Interleukin-3,IL-3 ELISA KIT 96T/48T
108932 人白介素4(IL-4) Human Interleukin-4,IL-4 ELISA KIT 96T/48T
108933 人的白介素-5(IL-5) Human Interleukin-5,IL-5 ELISA KIT 96T/48T
108934 人白介素6(IL-6) ELISA KIT Human Interleukin-6 ELISA KIT 96T/48T
108935 人白介素-8(IL-8)ELISA KIT Human Interleukin-8,IL-8 ELISA KIT 96T/48T
108936 人白介素-9(IL-9) Human Interleukin-9,IL-9 ELISA KIT 96T/48T
108937 人層連蛋白(Laminin) Human Laminin ELISA KIT 96T/48T
108938 人苗條素受體(Leptin receptor) Human Leptin receptor ELISA kit 96T/48T
108939 人瘦素(Leptin) Human Leptin ELISA kit 96T/48T
108940 人白血病抑制因子(LIF) Human Leukemia inhibitory factor, LIF ELISA kit 96T/48T
108941 人的L-Selectin Human L-Selectin ELISA kit 96T/48T
108942 人單核細(xì)胞趨化蛋白-1(MCP-1) Human Monocyte chemotactic protein-1,MCP-1 ELISA kit 96T/48T
108943 人單核細(xì)胞趨化蛋白-2(MCP-2) Human Monocyte chemotactic protein-2,MCP-2 ELISA kit 96T/48T
108944 人單核細(xì)胞趨化蛋白-3(MCP-3) Human Monocyte chemotactic protein-3,MCP-3 ELISA kit 96T/48T
108945 人巨噬細(xì)胞克隆刺激因子(M-CSF) Human Macrophage Colony-Stimulating Factor,M-CSF ELISA kit 96T/48T
108946 人巨噬細(xì)胞來源的趨化因子(MDC) Human Macrophage-Derived Chemokine,MDC ELISA KIT 96T/48T
108947 人巨噬細(xì)胞炎性蛋白-1α ( MIP-1α) Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit 96T/48T
108948 人巨噬細(xì)胞炎性蛋白-1β (MIP-1β) Human Macrophage Inflammatory Protein-1β,MIP-1β ELISA kit 96T/48T
108949 人巨噬細(xì)胞炎性蛋白-3α(MIP-3α) Human Macrophage Inflammatory Protein-3α,MIP-3α ELISA kit 96T/48T
108950 人巨噬細(xì)胞炎性蛋白-3β( MIP-3β) Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA kit 96T/48T
108951 人基質(zhì)金屬蛋白酶1(MMP-1) Human MMP-1——Matrix metalloproteinase-1 ELISA kit 96T/48T
將抗原或抗體固定在過程稱為包被(coating)。換言之,包被即是抗原或抗體結(jié)合到固相載體表面的過程,脂類物質(zhì)無法與固相載體結(jié)合,可將其在有機(jī)溶劑(例如乙醇)中溶解后加入板孔中,開蓋置冰箱過夜或冷風(fēng)吹干,待酒精揮發(fā)后,讓脂質(zhì)自然干固在固相表面??剐牧字贵w的ELISA試劑一般采用這種包被方式。
人趨化因子(fractalkine/CX3CL1)ELISA試劑盒
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